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Assay Kit
Zombie Aqua(TM) Fixable Viability Kit
Zombie Aqua(TM) Fixable Viability Kit from BioLegend
(3)
Write a review
Citations:
(641)
Supplier Page
Supplier Page from
BioLegend for
Zombie Aqua(TM) Fixable Viability Kit
Description
Zombie Aqua(TM) Fixable Viability Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Zombie Aqua(TM) Fixable Viability Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423102
Quantity
500 tests
Applications
Flow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (641)
(1)
AMPK activation induces immunogenic cell death in AML
Blood Advances
December 26, 2023
Johanna Mondesir, Margherita Ghisi, Laura Poillet, Robert A. Bossong, Oliver Kepp, Guido Kroemer, Jean-Emmanuel Sarry, Jérôme Tamburini, Andrew A. Lane
(
Cell Signaling Technology
, no.
4083S
); Zombie Green (
BioLegend
, no.
423111
); Zombie Aqua (
BioLegend
, no.
423101
); anti-
CALR
antibody, ER marker (Abcam, no. ab 2907); goat anti-rabbit IgG (H+L), secondary antibody
(2)
Profiling whole-tissue metabolic reprogramming during cutaneous poxvirus infection and clearance
Journal of Virology
December 21, 2023
Luxin Pei, Kirsten E. Overdahl, John P. Shannon, Katherine M. Hornick, Alan K. Jarmusch, Heather D. Hickman
ThermoFisher
56-0112-82
),
Gr-1
AF647 (
clone
: RB6-8C5,
Biolegend
108418
), and live-dead Zombie Aqua (
Biolegend
423102
) on ice for 30 minutes. After washing, cells were fixed with 3.2% paraformaldehyde (PFA) for 15 minutes
(3)
Single-cell characterization of human GBM reveals regional differences in tumor-infiltrating leukocyte activation
Elife
December 21, 2023
Philip Schmassmann, Julien Roux, Steffen Dettling, Sabrina Hogan, Tala Shekarian, Tomás A. Martins, Marie-Françoise Ritz, Sylvia Herter, Marina Bacac, Gregor Hutter
and subsequently washed with FACS buffer. To exclude dead cells Zombie Aqua Fixable Viability Kit (#
423102
,
1:100
,
BioLegend
) was added. Acquisition was performed on a CytoFLEX (Beckman). Data was analyzed using
(4)
LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy
Oncoimmunology
December 21, 2023
Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi Zhang
cell-surface
staining
, all samples were incubated with a Zombie Aqua Fixable Viability Kit (
BioLegend
, cat. #
423101
) for 15 min at room temperature. To detect cytokine production, cells were treated with a Cell Activation
(5)
Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models
Nat Commun
December 20, 2023
Jia Cao, Ling Jin, Zi-Qi Yan, Xiao-Kai Wang, You-You Li, Zun Wang, Yi-Wei Liu, Hong-Ming Li, Zhe Guan, Ze-Hui He,
et al.
scatter width (FSC-W) features, and dead cells were distinguished using Zombie Aqua dye (
Biolegend
,
423101
,
1:1000
). Equipment
Flow cytometry
analyses were performed on
FACSCanto
II Cell Analyzer (
BD Biosciences
)
(6)
Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment
Nat Commun
December 20, 2023
Xiaojian Lu, Yanwei Luo, Xichen Nie, Bailing Zhang, Xiaoyan Wang, Ran Li, Guangmin Liu, Qianyin Zhou, Zhizhong Liu, Liqing Fan,
et al.
previously reported using the
flow cytometer
(Cytec, USA)74,77. Zombie Aqua fixable viability kit (catalog:
423101
,
1:200
),
APC-anti-human CD19
(catalog:
302212
,
1:100
), PE-Cy7-anti-human 4-1BB (catalog:
309817
,
1:100
)
(7)
An epitope encoded by uORF of RNF10 elicits a therapeutic anti-tumor immune response
Molecular Therapy - Oncolytics
December 19, 2023
Lili Zeng, Wei Zheng, Jiahui Zhang, Jiawen Wang, Qing Ji, Xinglong Wu, Yaming Meng, Xiaofeng Zhu
were incubated for 5 h at 37°C, stained with Zombie Aqua Fixable Viability Kit (BV525,
BioLegend
, Cat#
423101
), anti-mouse CD8 (PerCP,
BioLegend
, Cat#
100732
), and anti-mouse CD3 (APC-Cy 7, BD
Pharmingen
, Cat#
(8)
Thrombocyte-derived Dickkopf1 promotes macrophage polarization in the Bleomycin-induced lung injury model
Front Immunol
December 15, 2023
Eun-Ah Sung, Min Hee Park, SuJeong Song, Hasan Alanya, Octavian Henegariu, Jinze Liu, E Zeynep Erson-Omay, Patricia J. Sime, Wook-Jin Chae
(
Miltenyi Biotec
). After blocking the Fc Receptor, cells were stained with Zombie Aqua Fixable Viability Kit (
423102
,
BioLegend
). Subsequently, the cells were stained with fluorescent-conjugated antibodies against different
(9)
Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicity
Front Immunol
December 8, 2023
Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin,
et al.
Fixable Viability Kit (
Biolegend
, Cat.:
423105
) Zombie Aqua™ Fixable Viability Kit (
Biolegend
, Cat.:
423101
), fixable viability dye e780 (eBioscience, Cat.: 65-0865) or the BD Horizon™
Fixable Viability Stain 700
(10)
Immune checkpoints are predominantly co-expressed by clonally expanded CD4+FoxP3+ intratumoral T-cells in primary human cancers
J Exp Clin Cancer Res
December 6, 2023
Delphine Bredel, Edi Tihic, Séverine Mouraud, François-Xavier Danlos, Sandrine Susini, Marine Aglave, Alexia Alfaro, Chifaou Mohamed-Djalim, Mathieu Rouanne, Héloise Halse,
et al.
(
Biolegend
,
372706
) mAbs were used for surface
labeling
. Zombie aqua fixable viability kit (
Biolegend
,
423102
) was used to exclude dead cells. Cells were then permeabilized with FoxP3/Transcription Factor Staining
(11)
Innate and Adaptive Cell-Mediated Immune Responses to a COVID-19 mRNA Vaccine in Young Children
Open Forum Infect Dis
December 2, 2023
Adriana Weinberg, Michael J Johnson, Krystle Garth, Elena W Y Hsieh, Ross Kedl, Daniela Weiskopf, Mattie Cassaday, Cody Rester, Berenice Cabrera-Martinez, Ryan M Baxter,
et al.
incubation
at 37 °C in a humidified 5% CO2 atmosphere, cells were stained with zombie aqua (
Biolegend
423101
) viability dye and monoclonal antibodies recognizing the following cell surface markers:
CD19
(BV605,
(12)
Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis
STAR Protocols
December 1, 2023
Enitome E. Bafor, Toni Martin, Jeffrey Carrell, Megan Karwan, Adrienne E. Kimmel, Uyi.G. Omogiade, Michael Sanford, Howard A. Young, Julio C. Valencia
90 μL of FACS buffer. Use immediately.•Viability dye (Zombie Aqua Fixable Viability Kit,
BioLegend
#
423102
).•RPMI medium.CRITICAL: Prepare FBS under sterile conditions. Filter the solution through a 75 mm rapid
(13)
Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours
Cancer Cell International
November 18, 2023
Ansooya A. Bokil, Mathieu Le Boulvais Børkja, Camilla Wolowczyk, Apsana Lamsal, Wenche S. Prestvik, Unni Nonstad, Kristine Pettersen, Sonja B. Andersen, Anna M. Bofin, Geir Bjørkøy,
et al.
the cell suspensions were washed twice, and samples were stained with Zombie Aqua (
BioLegend
, Cat #
423101
) for 10 min on ice, according to the manufacturer’s instructions. After surface
staining
, cells were
(14)
A rat liver cell atlas reveals intrahepatic myeloid heterogeneity
iScience
November 17, 2023
Delaram Pouyabahar, Sai W. Chung, Olivia I. Pezzutti, Catia T. Perciani, Xinle Wang, Xue-Zhong Ma, Chao Jiang, Damra Camat, Trevor Chung, Manmeet Sekhon,
et al.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Zombie Aqua™ Fixable Viability Kit,
BioLegend
,Cat#
423101
Mouse anti-rat CD45 BV786;
Clone
: OX-1,
BD Biosciences
,Cat#
740914
; RRID: AB_2740556 Mouse anti-rat
(15)
Mice with FVB-derived sequence on chromosome 17 succumb to disseminated virus infection due to aberrant NK cell and T cell responses
iScience
November 17, 2023
Taylor N. Tibbs, Lauren J. Donoghue, Ashlyn A. Buzzelli, Ichiro Misumi, Maggie DeMonia, Martin T. Ferris, Samir N.P. Kelada, Jason K. Whitmire
enzyme and buffer,
ThermoFisher Scientific
,Cat#
ER0911
Zombie Aqua Fixable Viability Dye,
Biolegend
,Cat#
423101
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Foxp3
Permeabilization/Fixation
(16)
Inadequate structural constraint on Fab approach rather than paratope elicitation limits HIV-1 MPER vaccine utility
Nature Communications
November 8, 2023
Kemin Tan, Junjian Chen, Yu Kaku, Yi Wang, Luke Donius, Rafiq Ahmad Khan, Xiaolong Li, Hannah Richter, Michael S. Seaman, Thomas Walz,
et al.
antibody (
Southern Biotech
,
2040-09
,
1:500
) mixed with Zombie Aqua Fixable viability dye (
BioLegend
,
423101
,
1:500
) at 4 °C for 30 min. Cell-surface
fluorescence
was analyzed by BD LSR Fortessa. For each
transfected
(17)
IL6 suppresses vaccine responses in neonates by enhancing IL2 activity on T follicular helper cells
npj Vaccines
November 8, 2023
Swetha Parvathaneni, Jiyeon Yang, Leda Lotspeich-Cole, Jiro Sakai, Robert C. Lee, Mustafa Akkoyunlu
splenocytes. Dead cells were stained by incubating cell suspensions with Zombie Aqua (
BioLegend
, Cat #
423102
) diluted at
1:1000
dilution in PBS for 15 min at room temperature. Cells were washed and stained using
(18)
Resveratrol Attenuates 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Mediated Induction of Myeloid-Derived Suppressor Cells (MDSC) and Their Functions
Nutrients
November 3, 2023
Wurood Hantoosh Neamah, Alex Rutkovsky, Osama Abdullah, Kiesha Wilson, Ryan Bloomquist, Prakash Nagarkatti, Mitzi Nagarkatti
Kits were purchased from StemCell Technologies (Vancouver, Canada). Zombie Aqua Fixable Viability Kit (
423102
) was purchased from
BioLegend
. The XFp glycolytic rate
assay
kit was purchased from Agilent Technologies
(19)
Acute irradiation causes a long-term disturbance in the heterogeneity and gene expression profile of medullary thymic epithelial cells
Frontiers in Immunology
November 2, 2023
Kenta Horie, Kano Namiki, Kyouhei Kinoshita, Maki Miyauchi, Tatsuya Ishikawa, Mio Hayama, Yuya Maruyama, Naho Hagiwara, Takahisa Miyao, Shigeo Murata,
et al.
Cat#
116512
),
FITC-anti-BrdU
(
BD
, BrdU flow kit, Cat#
559619
), Zombie Aqua fixable viability kit (
BioLegend
, Cat#
423101
), and anti-mouse CD16/32 (
BioLegend
,
clone
93, Cat#
101302
). Isolation and analysis of TECs from
mice
Mice
(20)
PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models
The Journal of clinical investigation
November 1, 2023
Baldrighi M, Doreth C, Li Y, Zhao X, Warner E, Chenoweth H, Kishore K, Umrania Y, Minde DP, Thome S,
et al
.
counter (Chemometec). Collected cell samples were then stained with Zombie Aqua viability dye (
BioLegend
,
423102
) for 10 minutes at room temperature in the dark. Then, samples were stained with the desired antibodies
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Reviews
Write a Review
(1)
12/5/2023
Viability Kit From BioLegend Requires Titration as It Provides Bright Signal
Samantha Chin
I have used other Zombie Viability dyes in the past and here have Zombie Aqua to stain for the viability of my tumor cells. Using Zombie Aqua, I was able to determine what percentage of my tumor cells...
Read Review
(1)
4/20/2021
Decent Fixable Viability Dye for Flow Cytometry
Vincent Giacalone
Human PBMCs were isolated from blood, cultured in various conditions and stained for analysis by flow cytometry
(1)
4/20/2020
Blue Zombies
Gregor Werba
Easy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
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Zombie Aqua(TM) Fixable Viability Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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